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The supernatant was then loaded on top of a 12 mL 1050% sucrose gradient (25 mM HEPES pH 7.5, 5 mM MgCl 2 , 0.1 M KCl, 2 mM DTT, 0.1 mg/mL cycloheximide) and spun in an ultracentrifuge at 35,000 RPM at 4 C for 2 h

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