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This process, using glutamate (Glu), cysteine (Cys), and glycine (Gly) as raw materials, depends on glutamate cysteine ligase (GCL) and glutathione synthetase (GS) catalysis, present only in the cytoplasmic matrix [23]

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For immunofluorescence, circular slides were placed in 24-well plates and were seeded with 1104 L02 cells/400 l DMEM per plate

It assures laboratories and researchers that they are working with the exact molecule required for the study