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Following a washing step, Alexa Fluor 594-conjugated goat anti-rabbit IgG (1:400, Molecular Probes, Waltham, MA, USA) and/or Alexa Fluor 488-conjugated goat anti-mouse IgG (1:400, Molecular Probes) were/was added as the secondary antibody and DAPI was used for nuclear staining
Hng dn s dng: Dng sau khi tm, lau kh cc vng c th Ly mt lng va , thoa u ln da, kt hp v nh thm thu nhanh hn
457 showed that pHLIP-Ku80 (), which was created by covalently conjugating pHLIPs with PNA targeting KU80 via disulfide bonds, could selectively reduce Ku80 expression under acidic conditions
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10.1038/nature08894 141 TolhurstG.HeffronH.LamY
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