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After pooling the fractions containing pure 3,4- cis -leucocyanidin ( 6 ), MOPSO was added to a final concentration of 10 mM, the pH was set to 6.2 using 5 N NaOH and the pooled fractions were rotary-evaporated to a concentration of 7 mM of 3,4- cis -leucocyanidin ( 6 ), quantified by HPLC-UV (280 nm) with an external calibration curve with 3,4- trans -leucocyanidin ( 14 )
its a powerful regulator
doi: 10.1530/acta.0.1280313 143 MeinhardtUNelsonAEHansenJLBirznieceVCliffordDLeungKCet al
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CST has been implicated in several brain functions such as learning and memory, regulation of sleep/wakefulness rhythms and it is suspected to have an anticonvulsant activity (for review, see de Lecea, 2008)
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