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For intracellular staining of hexokinase-1, cells were stained for extracellular markers, fixed in 4% FA at RT, and stained with Anti-Hexokinase 1 (Abcam ab184818) diluted 1:100 in PBS 0.1% Tween-20 at RT

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Funding This research was supported by the Beijing Natural Science Foundation (6212009), and the Reform and Development Project of Beijing Academy of Agricultural and Forestry Sciences (XMS202411)