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hESCs were cultured in hESC maintenance medium containing DMEM/F12 (Gibco, #11330-082), 20% knockout serum replacement (KOSR) (Gibco, #10828028), 1 mM non-essential amino acids (Gibco, #11140-050), 1% L-glutamine (Gibco, #25-005-CI), 0.1 mM -mercaptoethanol (Gibco, #21985023), and 10 ng/mL bFGF (R&D System, #2v33-FB/CF)
Neurological symptoms, including paraesthesia and cognitive changes, improve more gradually and may require six months or longer
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Ventura P, Panini R, Verlato C, Scarpetta G, and Salvioli G (2000)
