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Lentivirus was generated by transfecting HEK293T cells plated to roughly 60% confluency in a six-well dish with 250 ng of pMD2.G, 750 ng of psPAX2 and 1,000 ng of lentiviral vector containing the gene of interest with 10 l of PEI in serum-free medium
Patients should be encouraged to track symptoms, weight changes, and any new or worsening concerns
If you accidentally double-dosed, contact your healthcare provider immediately or call a poison control center
Soft-textured, protein-rich foods are often easier to manage when appetite is low: Small portions of wholegrains and legumes can support fibre intake without overwhelming the stomach and healthy fats such as olive oil, avocado and nuts add energy density without large volume, which can be useful when overall intake is modest