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For human islets, acute incubations were performed in KHB buffer at the indicated concentration of glucose agonist 28

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Differential expression analysis The Seurat FindMarkers function and the MAST package (v1.8.2) were employed for the calculation of differentially expressed genes (DEGs) with associated false discovery rate (FDR)-adjusted P -value and magnitude of change expressed in natural log of fold change ( lnFC ) for each cell type or subtype

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Less expensive than MRI, no radiation unlike X-rays