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Validation of differentially expressed genes (DEGs) by reverse transcription-quantitative PCR (RT-qPCR) The DEGs identified in our transcriptomic studies were validated by RT-qPCR using the primer sequences shown in Additional file 3
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- Temperature monitoring revealed that tissue 3 mm from the probe took 2.5 minutes to reach 20C and did not reach 40C, potentially limiting therapeutic effect
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