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Erythrocytes were lysed twice by adding DEPC-treated H 2 O and NaCl (8.8%), followed by centrifugation (450 x g, 6 min, 4C, Universal 320 R, Hettich, Tuttlingen, Germany)
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ZincHomocysteine binding in cobalamin-dependent methionine synthase and its role in the substrate activation: DFT, ONIOM, and QM/MM molecular dynamics studies
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