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After washing sessions in PBS for 3 10 min, these were then incubated in donkey Alexa-488 and 594 conjugated secondary antibodies (1:100 dilutions, Thermofisher, UK) for 1 h at RT
Razzaque, Lake Erie College of Osteopathic Medicine, United States Reviewed by Naseh Pahlavani, Torbat Heydarieh University of Medical Sciences, Iran
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It has been reported that the commercially available antibodies are thought to be nonspecific or inaccurate for GLP-1R protein detection 33
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