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(SEMALCO)
it is registered as a dietary supplement (NR precursors have GRAS status in the USA)
Notably, phosphorylation was decreased significantly in PRMT7 T73A mutant and this T73 residue was highly conserved across species (Fig
Membranes were probed with primary antibodies overnight on a 4C shaker and then incubated with horseradish peroxidase (HRP)conjugated secondary antibodies, and signals were visualized with ECL (BioRad)
Alexa 488- or CY3-conjugated secondary antibodies (Thermo Fisher Scientific, Waltham, MA, United States, 1:200 dilution) were diluted to 1:300
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