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Cell R software (Olympus Soft imaging solutions, Munster, Germany) or WinFluor Fluorescence Image Capture and Analysis program (John Dempster, University of Strathclyde, Scotland) was used to record excitation changes in intracellular calcium (OMalley et al., Figure 2 Figure 3 As previously described (OMalley et al., 2+ ] i ) that was greater than two standard deviations from the baseline values in that cell (calculated during the 150 s preceding stimulus application)
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Our tests showed that 7 out of 14 dark chocolate bars had high cadmium, as did 2 of 9 popular cocoa powders
Omega-3 fatty acids (3-4g daily), niacin (if tolerated), regular cardiovascular exercise, and limiting saturated fat intake all help maintain healthier lipid levels during cycles
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