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RNA isolation, cDNA synthesis, and quantitative RT-PCR H9c2, NMCMs, and hiPSC-CMs were collected into QIAzol Lysis Reagent or Trizol Reagent (Invitrogen) and total RNA was extracted using Direct-zol RNA Miniprep and Microprep (Zymo Research, CA, USA), respectively

Knee pain types were unspecified

In this situation, adipocytes encountering high insulin and low GH levels can only store nutrients and not start lipolysis, the ideal situation for gaining weight instead of losing it

Moreover, patients may struggle to articulate ISP, leading to underreported discomfort