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At 7 dpi, the wells were fixed with 2 mL/well of 7% Formaldehyde (VWR,10790-708) for 30 min and with 0.5 mL/well of 1x crystal violet (VWR, 470337-534) for 15 min

In APP/PS1 Alzheimer's mice, Dihexa treatment not only prevented cognitive decline but actively restored spatial learning abilities that had already been compromised, indicating the compound promotes recovery of function in damaged neural circuits