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Test Spot Requirement SC: Initial 2.5mg test dose, monitor for 30 minutes for any reactions before full dose

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doi:10.1038/nm.2513 255

To determine whether TBB inhibition preferentially triggers apoptosis of senescent cells, Zmpste24 / MEFs treated with TBB were stained for C 12 FDG to label senescent cells, then stained for Annexin V, an early apoptosis marker, and 7-aminoactinomycin D (7-AAD), a membrane impermeable dye that is generally excluded from viable cells as a later apoptosis marker, and finally analyzed with flow cytometry