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In another clinical trial, patients with advanced breast cancer received cyclophosphamide, 5-FU, and epirubicin (Ellence) along with a DHA supplement

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On day 6 of differentiation, the medium was changed to glucose/phenol red/glutamine/pyruvate-free DMEM (Thermo Fisher Scientific) supplemented with 0.5% FCS, 4.5 g l 1 glucose, 4 mM glutamine, 0.5 mM pyruvate and 5 g ml 1 insulin