Use needles of shorter length (4 mm or the shortest available) and smaller diameter (highest gauge number), and the tip with the lowest penetration force to minimize pain
Male C57BL/6 J mice (810 weeks, Charles River) were maintained at 2123 C and light-dark cycles (12:12 h schedule, lights on at 07:00)
FAP-AF700 (R&D Systems, FAB3715N, clone 427819, lot AEVI020011, FCy, 1:100, Hu)
The relatively short duration and transient nature of vomiting episodes suggest that most cases resolve quickly as patients adapt to the medication or implement dietary modifications
As with any medication, Semaglutide GLP-1 may not be suitable for everyone and can cause side effects such as nausea, diarrhea, vomiting, and the like