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For the second time point, add 100l of the 1:1 mixture of diamide and 2-DG into the first petri dish, such that the final concentration of diamide and 2-DG is 25mM each

This makes the math for calculating doses very straightforward (e.g., 0.1mL for a 50mcg dose), which helps minimize the risk of calculation errors during daily lab work

C., & Nuss, J

Glutathione-S transferase activity in cultured research subjects hepatocytes [PMID: 3228864] Laboratory literature summary: Glutathione-S transferase activity in cultured research subjects hepatocytes