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Even in small amounts vitamin C can protect indispensable molecules in the body, such as proteins, lipids (fats), carbohydrates, and nucleic acids (DNA and RNA), from damage by free radicals and reactive oxygen species (ROS) that are generated during normal metabolism, by active immune cells, and through exposure to toxins and pollutants (e.g., certain chemotherapy drugs and cigarette smoke)
Z., Loizidou, M., Ahmed, M
properties of a good affinity tag binds something that can be attached to little beads (resin) that bindings reversible that bindings specific (only tagged proteins bind it) that bindings high-affinity (your protein sticks & stays stuck until youre ready to remove it after washing the other stuff off) To make sure the tag doesnt interfere with our protein, we can tether it with a flexible linker and we can design the linker to have a sequence that a site-specific endoprotease (protein scissors) will recognize and cut at so we can cut the tag off after its served its purpose
& Vitalone, A