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There can be a maintenance period

Measurement of serum and liver antioxidant indexes 0.2 g of liver tissue was weighted and homogenized in 2 mL of physiological saline, following by centrifugation at 3,500 g for 15 min and the lower solvent layer was obtained as liver homogenate

When supplementation stops, glutathione levels may gradually return to baseline depending on diet, lifestyle, and overall health.