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Researchers block free GSH using alkylating agents such as 2-vinylpyridine (2-VP) or N-ethylmaleimide (NEM) before running the assay, which isolates GSSG for independent measurement

Gallstones form when bile components harden into stone-like deposits in the gallbladder or bile ducts, causing inflammation and pain

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It functions as a major intracellular antioxidant and plays a critical role in cellular detoxification, oxidative stress reduction, and immune support

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