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BSO treatment of HeLa cells reduces the level of 14S pentamers and alters the mobility and stability of 150S particles With the aim of determining which step of particle assembly is inhibited by BSO we applied 35 S-labelled PV1-wt infected HeLa cell lysates, made in BSO treated or untreated cells, to sucrose gradients to separate the 5S, 14S, 75S and 150S fractions (Figures 7A and 7B)
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