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In Annals of Pharmacotherapy
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However, restoration of mTOR, p70S6K, or 4E-BP1 was remarkably potent in protecting against neuronal apoptosis caused by these PD mimetics 10,15
The mixed solution was stirred at room temperature for 24 h and the precipitate was collected by centrifugation at 12000 rpm for 10 min, followed by gently washing 3 times with DI water to remove the free BSO
Lyophilized powder freezes fine for long-term storage, but once a peptide is in solution, ice crystals physically damage the structure you lose potency and get precipitation on thaw
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