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Cells or islets were fixed at room temperature for 20 minutes in 2% PFA + 2% glutaraldehyde in 0.1 M sodium cacodylate, washed 2x with 0.1 M sodium cacodylate and 1x with ddH 2 O, and then post-stained on ice with 2% osmium tetroxide for 1 hour followed by 1% tannic acid at room temperature for 45 minutes, and washed 2x in 0.05 M sodium cacodylate before stepwise dehydration in 70%, 90%, and 100% EtOH and Epon resin embedding