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5-fluorouracil: mechanisms of action and clinical strategies
For experiments in 384-well plates, wells were aspirated, rinsed with PBS and incubated with a fixative and staining solution containing 3.7% formaldehyde (Fisher Scientific, F75P-1GAL) and 5 g ml 1 Hoechst 33342, trihydrochloride, trihydrate (Invitrogen, H1399)
G.MonteiroW
This was followed by 12 cycles of denaturation at 98 C for 10 s and annealing at 60 C for 30 s
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