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Total DNA was extracted using 20 mM Tris pH 8.0, 5 mM EDTA, 400 mM NaCl, 1% SDS and 40 g/mL proteinase K, as previously described 109 , and used at 3 ng/mL for qPCR analysis of mitochondrial DNA (mtDNA) relative to genomic DNA (gDNA) as in 110
Choose the ratio first, because it changes the per-unit math
Besides, previous studies in mice models of AD showed that linagliptin could decrease A accumulation, attenuate tau phosphorylation and inhibit neuroinflammation (83)
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It solved the puzzle of GLP-1s short half-life, offering a path towards therapeutic application
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