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In smoke exposure or elastase-induced emphysema models, Nrf2 activation can alleviate oxidative stress, inflammatory infiltration, and alveolar structural damage, and some studies suggest that it can downregulate NLRP3 inflammasome activation and IL-1/IL-18 release, thereby indirectly inhibiting necroptosis (291, 292)

This indicates that cells, and specifically mitochondria, that suffer from deuterium overload would be more susceptible to oxidative stress mediated by H 2 O 2

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Moreover, to validate the results, the experimental procedures were repeated three times at a 2-week interval in the same conditions