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In the Phase IIb trial data summarized by Metabolic Pharmaceuticals, participants receiving up to 1 mg/day of oral AOD-9604 for 24 weeks showed no statistically significant changes in fasting glucose, insulin, or IGF-1 compared to placebo [6]
The cells were collected 24 h after transfection, digested with EDTA-free trypsin, washed twice with pre-cooled PBS and placed in 70% ethanol at -20 C overnight
doi: 10.1210/jcem-69-4-745 72 HakunoFTakahashiSI
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Cell transfection The endotoxin-free plasmids were prepared with plasmid miniprep plus purification kit (DP01-Plus-300, GeneMark), purified by ethanol precipitation, and subjected to cell transfection
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