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After incubation for 1 h, the DCFH-DA probe (10 M) was added and incubated in the dark for 30 min to detect ROS

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We think that the [neurons influence] on intervertebral disc degeneration is not only limited to the occurrence of pain, but also intervertebral disc degeneration itself, said Ximing Xu, professor in the Department of Orthopedic Surgery in Changzheng Hospital, and co-lead author of the study

Using this system, the excretion of protein-bound uremic toxins and reabsorption of albumin were shown under perfusion conditions, thus mimicking the in vivo situation (Jansen et al