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Immunofluorescence using the conformation-specific antibody 9EG7, which recognizes active 1 integrins 37 , showed that all ectopically expressed human 1 integrins (no-tag, ecto-tag, and cyto-GFP) co-localized with the FA marker vinculin, just like endogenous mouse 1 integrin in parental fl/fl cells (Fig

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