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doi: 10.7759/cureus.54045 201 TripathiAPandeyVKMishraHDarAHSinghGRustagiSet al

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2.8 Real-time quantitative PCR Total RNA was isolated from BMSCs cultivated under various conditions employing TRIzol reagent (Ambion, United States), and cDNA was synthesised employing the cDNA HiFiScript Synthesis Kit (Cwbio, China)

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