l carnitine neurotransmitter Acetyl-L-carnitine physical-chemical, metabolic, and therapeutic properties: relevance for its mode of action in Alzheimer's disease and geriatric depression The protective role of l-carnitine

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After centrifugation (16,700 rpm, 15 min, Beckman SW-40Ti rotor), the fraction between the 22% and 50% Percoll gradients containing intact mitochondria was carefully transferred into a new reaction tube, mixed with 2 volumes of HBS buffer, and pelleted by centrifuging at 20,000 g for 20 min at 4 C to collect the mitochondrial samples for further analyses

The analytical data obtained were found to be consistent with the existing literature 48

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