Carefully, lead the needle tip into the outer needle cap without touching the needle (See Figure K) and push the outer needle cap completely on
the coupling agent used in the reaction can be selected from group consisting of [Ethylcyano (hydroxyimino)acetate-02)-tri-(l-pyrrolidinyl)-Phosphonium hexa fluorophosphate (PyOxim), ethyl-2 -cyano-2-(hydroxy amino) acetate (Oxyma pure), 0-(benzotriazol-l-yl)-N,N,N,N'- tetramethyluronium tetrafluoroborate (TBTU), diisopropyl carbodiimide (DIC), 1,3- dicyclohexylcabodiimide (DCC) , 0-(7 -azabenzotriazol- 1 -yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HATU), 1 -(dimethyl aminopropyl)-3-ethylcarbodiimide hydrochloride (ED the reaction temperature may range from 25C to 30C
Recovery can take six to eight weeks or more, depending on the type and extent of the surgery
Soto-Heras S, Paramio M
Patients should be monitored for signs and symptoms of acute gallbladder disease