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Cossins, E., Lee, R

(1843-1898), then a lecturer in clinical medicine at the University of Pennsylvania in Philadelphia (and subsequently Professor of Clinical Medicine, Professor of the Theory and Practice of Medicine, and the longtime Provost of the university), noted the extreme hyperplasia of the bone marrow in patients with pernicious anemia (139)

The cell pellets were sonicated (peak power: 75, duty factor: 20, cycle: 200) and then transferred to a new tube and centrifuged at full speed for 20 min at 4 C

By 1955, British chemist Dorothy Crowfoot Hodgkin (1910-1994) of Cambridge University determined the molecular structure of cyanocobalamin using computer-assisted x-ray crystallography, work for which she received the 1964 Nobel Prize in Chemistry (139)