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Cell culture and drug treatment SH-SY5Y cells were obtained from ATCC (Manassas, VA, USA) and cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, at 37 C in a humidified atmosphere with 5% CO 2
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Moreover, elevated seminal IL-1 levels are strongly associated with decreased mitochondrial activity in sperm, suggesting that pro-inflammatory cytokines not only originate from testicular inflammation but also act within the seminal microenvironment to damage mature sperm (73)
Sample Handling in Turku University Hospital Auria BioBank Pathology Collection All blood samples were taken from BioBank storage after given informed consent from patients
He notes that this higher dose is balanced by his diluted formula
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