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The obtained supernatant was collected in Eppendorf and used for biochemical estimation and Pellets, (containing mitochondria of interest) were re-suspended in isolation buffer and mitochondrial solutions were centrifuged at 4 C for 10 min at 10,000 g
Both in vitro and in vivo assay demonstrated that METTL14 induced N6 -methyladenosine (m 6 A) modification at 5UTR of SLC7A11 mRNA, which promotes SLC7A11 mRNA stability and upregulates its expression by inhibiting the deadenylation process, enhancing ferroptosis resistance (Fan et al., 2021
It also supports detoxification by binding to toxinssuch as heavy metals, pesticides, and environmental pollutantsand facilitating their removal
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