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Incubations of the cells were carried out in microplates at a density of 1.5 10 4 cells per well in HBSS buffer (Invitrogen) with 20 mM HEPES (pH 7.4) and 500 M IBMX
In addition, elderly, obese, hypertensive and frail populations have been reported to have lower plasma L-carnitine levels 9 ( Figure 1 )
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