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Following three PBS washes (5 min each), the sections were incubated for 30 min at room temperature with biotinylated secondary antibody and avidinbiotin complex (Vectastain ABC-peroxidase kit, Vector Laboratories, Burlingame, CA)

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Cell viability was assessed using the Cell Counting Kit-8 (CCK8) assay, measuring metabolic activity as an indicator of cell health

from 2004 suggests that under continuous 24-hour evaluation, GHRP-2 may also increase growth hormone production from approximately 2030 gL24 h in placebo controls to about 120180 gL24 h with GHRP-2, suggesting an estimated 4- to 6-fold increase