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The stock solution of trace elements contained the following in 3 M HCl: FeSO 4 7H 2 O 80, AlCl 3 6H 2 O 10, ZnSO 4 7H 2 O 2.0, CuCl 2 2H 2 O 1.0, NaMoO 4 2H 2 O 2.0, MnSO 4 nH 2 O 10, CoCl 2 4.0, and H 3 BO 4 0.5 g/L
Three days after seeding of micromass cultures and treatment with various concentrations factors of interest, wells were washed once with phosphate-buffered saline and then fixed for 20 min with 1 mL 10% neutral buffered formalin
For insulin syringes, this is approximately 0.5 to 1 unit
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