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The lentiviral particles for CBS OPT and L2HGDH OPT constructs were generated similarly with the packaging system as those for sgRNA but selected with puromycin at a final concentration of 1.5 g/ml
Example 1 Step 1: weighing injection water with the prescription amount of about 30 percent, cooling to 20-30 , and filling high-purity nitrogen into the injection water to replace oxygen in the solution until the oxygen content in the solution is less than 1.5 ppm
The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
the newly formed acylcarnitine is then exported into the cytosol via CACT
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