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Animals were sacrificed at 0.5, 1, 4, 24 hours and 1, 2, and 3 weeks after TNBS+ethanol administration and colonic mucosa was obtained for evaluation of damage, GSH content, total RNA isolation and protein content
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Nu mt protein tip xc vi nhit cao hn nhit m n c th chu ng, n s to ra menggumpal
After incubation with the secondary antibody, the cells were washed with PBST and mounted using a Fluoroshield Mounting Medium with DAPI (Abcam ab104139)
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