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Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) GAPDH catalyzes a redox reaction, forming a high energy acyl phosphate compound To set the stage for substrate-level phosphorylation, the aldehyde group of glyceraldehyde-3-phosphate is oxidized to a carboxylic acid and the energy available from the oxidation reaction is used, in part, to trap a phosphate from the cytoplasmic pool as an acyl phosphate
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Consequently, it is difficult to avoid hypothesising the existence of niche-specific host-microbe interactions that are functional for tissue memories in epithelial barriers such as skin
After blocking in PBS with 0.1% BSA at room temperature for 10 min, cells were stained with the following antibodies: anti-CD117 (c-Kit, Biolegend), anti-Ly-6A/E (Sca-1, Biolegend), anti-CD150 (SLAM, Biolegend), anti-CD135 (Flk-2, Biolegend), anti-CD34 (Biolegend), and/or FITC anti-mouse Lineage Cocktail (Biolegend)
