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Insert the needle at a 90-degree angle to the skin surface

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SARS-CoV-2-infected patients with COVID-19 disease show alterations in the glucoseinsulin axis leading to hyperglycemia, hyperinsulinemia and insulin resistance, increased oxidative/nitrosative stress, and significantly decreased vitamin D, thiols, total-antioxidant-capacity, GSH and selenium (Soto et al., 2022)

The brain slices were then incubated in the blocking buffer with the secondary antibody that was conjugated with the fluorochrome at room temperature for 2 hours