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Filter-sterilize through a 0.22 m low-protein-binding filter before introduction into sterile cell culture, and prepare working dilutions immediately before use

MAPK/ERK drives proliferation

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The cytoprotection concept after Robert and Szabo is extended systemically via modulation of the NO system Sikiric et al., 2025

Similarly, although fluorescent probes targeting ACSL4a key regulator of secondary ferroptosis in TBIachieve specific labeling in vitro , they leak out of injured brain tissue in vivo due to bloodbrain barrier disruption, limiting clinical utility