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Quantitative Real-Time Polymerase Chain Reaction (PCR) Quantitative real-time PCR reaction was performed using Rotor-Gene Q real time PCR cycler (Qiagen, Germany)

Traditionally made bone broth also contains a surprising amount of protein, between 8 and 12 grams per serving, depending on the brand

There was marked enrichment according to this analysis in glycolysis/gluconeogenesis, amino acid biosynthesis, carboxylic acid metabolism, and oxoacid metabolism

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