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At present, the WENR protocol has been widely used in the culture of many organoids, such as those from the stomach, small intestine, colon, pancreas and liver
Factors such as acidosis and the use of central nervous system (CNS) stimulants and depressants can influence the CNS levels of lidocaine HCl and limit its efficacy
Others find evening dosing more convenient without apparent difference in results
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The practical takeaway is unromantic
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