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Moreover, the use of GST 4QN as a tag reduced fused p53 aggregation and enhanced the induction of p21 transcription and apoptosis in cells treated with 5-fluorouracil (5-FU)
Real-Time PCR Assay For G6PD, NRF2 and NP mRNA quantification, total RNA was isolated from cell lysates (Total RNA Purification Plus Kit, Norgen Biotek, Thorold, ON, Canada) and used as a template for generating cDNA (iScript cDNA Synthesis Kit, Bio-Rad, Milan, Italy)
If the powder does not dissolve immediately, place it in the refrigerator for 30 minutes it will dissolve on its own
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