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For Western blot analysis, proteins were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to a polyvinylidene difluoride (PVDF) membrane

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GRK2+/ mice showed higher plasma insulin concentrations than WT littermates 10 min (early phase) but not 4 h (late phase) after ad libitum feeding (Fig