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Despite a large number of studies, the mechanism of toxicity of selenium has not been fully understood, and the current level of knowledge indicates that the toxicity of selenium may be the result of various mechanisms
Studies on the use of non-denaturing agents such as N -lauryl sarcosine, dimethyl sulfoxide (DMSO), 5% n -propanol, mild non-ionic detergents, high pH buffers, and low denaturant concentration that preserve the native-like state of the fusion proteins have been reported [52,53,54,55,56,57]
The choice between them affects stability and storage duration, not the calculation
10.1016/S0140-6736(18)32261-X 134
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